rat icam 1 elisa kit  (R&D Systems)

Journal: Journal of Cell Communication and Signaling

Article Title: Stent treatment improves cerebral microcirculatory disorder and blood–brain barrier function in internal carotid artery stenosis via intercellular adhesion molecule 1 modulation

doi: 10.1002/ccs3.70058

Figure Lengend Snippet: Transcriptomic analysis of rat internal carotid artery severe stenosis model before and after stent implantation. (A) Experimental flowchart illustrating the collection of brain tissue samples and transcriptomic analysis process before and after stent implantation in rats; (B) differential gene expression analysis results, showing the gene expression differences between brain tissue samples before and after stent implantation; (C, D) clusterProfiler enrichment analysis results, displaying the enrichment of DEGs in gene ontology and Kyoto encyclopedia of genes and genomes pathways; (E) correlation analysis between ICAM1 and genes related to inflammation, BBB function, and cerebral microcirculation; (F) protein–protein interaction network analysis results, demonstrating the central role of ICAM1 in the network and its interactions with other inflammation‐related proteins; (G) gene set enrichment analysis enrichment analysis results, indicating significant enrichment of the NF‐κB signaling pathway in DEGs after stent implantation; (H, I) western blot results, showing the nuclear translocation of NF‐κB p65 and changes in the expression levels of ICAM1 and IκBα after stent implantation. All data are presented as mean ± standard error. Experiments were repeated three times, and statistical analysis was performed using ANOVA with Tukey post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. DEGs, differentially expressed genes.

Article Snippet: ELISA assays for TNF‐α (ab236712, Abcam, UK), IL‐6 (ab234570, Abcam, UK), MMP‐9 (R6000B, R&D Systems, USA), and ICAM‐1 (RIC100, R&D Systems, USA) were implemented as per the manufacturers' protocols.

Techniques: Gene Expression, Western Blot, Translocation Assay, Expressing

Journal: Journal of Cell Communication and Signaling

Article Title: Stent treatment improves cerebral microcirculatory disorder and blood–brain barrier function in internal carotid artery stenosis via intercellular adhesion molecule 1 modulation

doi: 10.1002/ccs3.70058

Figure Lengend Snippet: Protective effects of ICAM1 knockdown on HBMEC and SH‐SY5Y cells under OGD conditions. (A) Schematic diagram of the experimental procedure, showing the experimental design for ICAM1 knockdown and OGD treatment; (B) western blot analysis of ICAM1 expression in HBMECs; (C) detection of reactive oxygen species levels in HBMECs using DCF‐DA fluorescence probe; (D) time‐dependent changes in transendothelial electrical resistance (Ω▪cm 2 ) values of HBMECs over 90 min; (E) immunofluorescence staining of tight junction proteins (ZO‐1 and occludin) in HBMECs; (F) RT‐qPCR analysis of antioxidant‐related genes (Nrf2 and HO‐1) expression in HBMECs; (G) RT‐qPCR analysis of inflammatory cytokines (TNF‐α and IL‐6) expression in HBMECs. All data are presented as mean ± standard error of the mean. Experiments were repeated three times, and statistical analysis was performed using ANOVA followed by Tukey's post‐hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. HBMEC, human brain microvascular endothelial cell; OGD, oxygen‐glucose deprivation; RT‐qPCR, reverse transcription quantitative polymerase chain reaction.

Article Snippet: ELISA assays for TNF‐α (ab236712, Abcam, UK), IL‐6 (ab234570, Abcam, UK), MMP‐9 (R6000B, R&D Systems, USA), and ICAM‐1 (RIC100, R&D Systems, USA) were implemented as per the manufacturers' protocols.

Techniques: Knockdown, Western Blot, Expressing, Fluorescence, Immunofluorescence, Staining, Quantitative RT-PCR, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Journal of Cell Communication and Signaling

Article Title: Stent treatment improves cerebral microcirculatory disorder and blood–brain barrier function in internal carotid artery stenosis via intercellular adhesion molecule 1 modulation

doi: 10.1002/ccs3.70058

Figure Lengend Snippet: Effect of ICAM1 knockdown on HBMEC function. (A) Schematic diagram of the experimental procedure, illustrating the experimental design for ICAM1 knockdown and oxygen–glucose deprivation treatment in HBMEC and SH‐SY5Y cells; (B) CCK‐8 assay to assess HBMEC cell proliferation; (C) Transwell migration assay to evaluate HBMEC cell migration capacity; (D) scratch assay to measure the migration rate of HBMEC cells; (E) tube formation assay to assess the in vitro angiogenic ability of HBMECs; (F) annexin V‐FITC/PI flow cytometry to determine the apoptosis rate of HBMEC cells. Data are presented as mean ± standard error of the mean. All experiments were performed in triplicate, and statistical analysis was conducted using ANOVA followed by Tukey's post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. HBMEC, human brain microvascular endothelial cell.

Article Snippet: ELISA assays for TNF‐α (ab236712, Abcam, UK), IL‐6 (ab234570, Abcam, UK), MMP‐9 (R6000B, R&D Systems, USA), and ICAM‐1 (RIC100, R&D Systems, USA) were implemented as per the manufacturers' protocols.

Techniques: Knockdown, CCK-8 Assay, Transwell Migration Assay, Migration, Wound Healing Assay, Tube Formation Assay, In Vitro, Flow Cytometry

Journal: Journal of Cell Communication and Signaling

Article Title: Stent treatment improves cerebral microcirculatory disorder and blood–brain barrier function in internal carotid artery stenosis via intercellular adhesion molecule 1 modulation

doi: 10.1002/ccs3.70058

Figure Lengend Snippet: Effects of stent implantation on cerebral microcirculation, cognitive function, and BBB in rats with severe internal carotid artery stenosis. (A) Diagram illustrating experimental groups and protocol; (B) laser speckle contrast imaging measurement of cerebral blood flow in rats; (C) Morris water maze test to assess spatial learning and memory in rats; (D) balance beam test to evaluate motor coordination in rats; (E) H&E staining to observe pathological changes in rat brain tissue structure; (F) western blot analysis of the expression levels of BBB tight junction proteins occludin and ZO‐1 in rat brain tissue; (G, H) ELISA measurement of inflammatory cytokines (TNF‐α, IL‐6) and the expression levels of ICAM1 and MMP‐9 in rat brain tissue. Data are presented as mean ± standard error of the mean, with experiments repeated three times and 10 rats per group. Statistical analysis was performed using ANOVA followed by Tukey's post‐hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: ELISA assays for TNF‐α (ab236712, Abcam, UK), IL‐6 (ab234570, Abcam, UK), MMP‐9 (R6000B, R&D Systems, USA), and ICAM‐1 (RIC100, R&D Systems, USA) were implemented as per the manufacturers' protocols.

Techniques: Imaging, Staining, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Nutrition

Article Title: In vivo effects of javamide-I/-II on metabolic, hepatic, cardiovascular and inflammatory risk factors

doi: 10.3389/fnut.2025.1661468

Figure Lengend Snippet: Effects of J1/J2 on sICAM and sE-selectin. sICAM (A) and sE-selectin (B) were measured using plasma samples. There were no significant differences in sICAM and sE-selectin levels between the CG and JG groups. Data is presented as the mean ± SD ( n = 10).

Article Snippet: Rat soluble intercellular adhesion molecule 1 (sICAM) level was determined in plasma samples using sICAM ELISA kit (Cat # RIC100, R&D systems, Minneapolis, MN, the United States) according to the manufacturer’s instructions.

Techniques: Clinical Proteomics